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cdna tick samples  (GenScript corporation)
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GenScript corporation cdna tick samples
Primers sequences used to amplify the <t> Bm86 cDNA </t> by rt-PCR
Cdna Tick Samples, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Primers sequences used to amplify the Bm86 cDNA by rt-PCR

Journal: BMC Genetics

Article Title: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns

doi: 10.1186/s12863-019-0754-8

Figure Lengend Snippet: Primers sequences used to amplify the Bm86 cDNA by rt-PCR

Article Snippet: From 150 Bm86 cDNA tick samples sequenced by Genscript (corresponding to 600 amplicons sequenced), only 125 could be successfully assembled for the complete putative Bm86 cDNA sequence.

Techniques: Sequencing

Genetic variability of the Bm86 gene in the different sampled localities

Journal: BMC Genetics

Article Title: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns

doi: 10.1186/s12863-019-0754-8

Figure Lengend Snippet: Genetic variability of the Bm86 gene in the different sampled localities

Article Snippet: From 150 Bm86 cDNA tick samples sequenced by Genscript (corresponding to 600 amplicons sequenced), only 125 could be successfully assembled for the complete putative Bm86 cDNA sequence.

Techniques:

Haplogroups for sequence Bm86 from Mexican R. microplus

Journal: BMC Genetics

Article Title: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns

doi: 10.1186/s12863-019-0754-8

Figure Lengend Snippet: Haplogroups for sequence Bm86 from Mexican R. microplus

Article Snippet: From 150 Bm86 cDNA tick samples sequenced by Genscript (corresponding to 600 amplicons sequenced), only 125 could be successfully assembled for the complete putative Bm86 cDNA sequence.

Techniques: Sequencing

Mantel test, scatter plot showing correlation between genetic and geographic distances of the Bm86 haplotypes from ticks collected in different areas of the Mexican Republic. Real values for matrices are observed. a , gene distances in percentages of similarity; b , geographical distances in kilometers

Journal: BMC Genetics

Article Title: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns

doi: 10.1186/s12863-019-0754-8

Figure Lengend Snippet: Mantel test, scatter plot showing correlation between genetic and geographic distances of the Bm86 haplotypes from ticks collected in different areas of the Mexican Republic. Real values for matrices are observed. a , gene distances in percentages of similarity; b , geographical distances in kilometers

Article Snippet: From 150 Bm86 cDNA tick samples sequenced by Genscript (corresponding to 600 amplicons sequenced), only 125 could be successfully assembled for the complete putative Bm86 cDNA sequence.

Techniques:

Minimum expansion network . It shows the frequency and relationship among haplotypes and haplogroups. Haplotypes and haplogroups are indicated with a colored label. Nine haplogroups can be observed. Haplogroups 1 and 2, containing the most abundant haplotypes. Haplotypes 10 to 64 are unique and have a random distribution in the network. GenBank Bm86 sequences included in the network come from Thailand (TH), Argentina (ARG), Mozambique (MOZ), United States of America (USA, TX), Mexico (MX), Brazil (BRA) and Australia (vaccine strain Yeerongpilly; YEERO)

Journal: BMC Genetics

Article Title: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns

doi: 10.1186/s12863-019-0754-8

Figure Lengend Snippet: Minimum expansion network . It shows the frequency and relationship among haplotypes and haplogroups. Haplotypes and haplogroups are indicated with a colored label. Nine haplogroups can be observed. Haplogroups 1 and 2, containing the most abundant haplotypes. Haplotypes 10 to 64 are unique and have a random distribution in the network. GenBank Bm86 sequences included in the network come from Thailand (TH), Argentina (ARG), Mozambique (MOZ), United States of America (USA, TX), Mexico (MX), Brazil (BRA) and Australia (vaccine strain Yeerongpilly; YEERO)

Article Snippet: From 150 Bm86 cDNA tick samples sequenced by Genscript (corresponding to 600 amplicons sequenced), only 125 could be successfully assembled for the complete putative Bm86 cDNA sequence.

Techniques:

Molecular Phylogenetic analysis by Maximum Likelihood method. Tree reconstruction of evolutionary distances among putative sequences of the Bm86 gene of Mexican tick populations was inferred by using the Maximum Likelihood method based on the Kimura 2-parameter model. A discrete Gamma distribution was used to model evolutionary rate differences among sites (2 categories (+G, parameter = 0.0500)). Bootstrap evaluation adjusted for 1000 permutation repetitions. The main clades considered here are shown in brackets marked as A and B. Clade B has 5 subclades labeled from B1 to B5. A total of 165 complete sequences, considering amino acid residues from 20 to 627 from the Bm86 were used in the analysis, including 125 Mexican sequences (localities are marked with circles of different colors) and GenBank sequences: 14 from Texas (USA, orange triangles); 18 from Thailand (brown triangles); 1 from Australia (blue triangle); 1 from Argentina (light blue triangle); 1 from India (pink triangle); 1 from Brazil (yellow triangle); the only previous Mexican (green triangle) and 2 Mozambique sequences used as outgroup (black triangles)

Journal: BMC Genetics

Article Title: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns

doi: 10.1186/s12863-019-0754-8

Figure Lengend Snippet: Molecular Phylogenetic analysis by Maximum Likelihood method. Tree reconstruction of evolutionary distances among putative sequences of the Bm86 gene of Mexican tick populations was inferred by using the Maximum Likelihood method based on the Kimura 2-parameter model. A discrete Gamma distribution was used to model evolutionary rate differences among sites (2 categories (+G, parameter = 0.0500)). Bootstrap evaluation adjusted for 1000 permutation repetitions. The main clades considered here are shown in brackets marked as A and B. Clade B has 5 subclades labeled from B1 to B5. A total of 165 complete sequences, considering amino acid residues from 20 to 627 from the Bm86 were used in the analysis, including 125 Mexican sequences (localities are marked with circles of different colors) and GenBank sequences: 14 from Texas (USA, orange triangles); 18 from Thailand (brown triangles); 1 from Australia (blue triangle); 1 from Argentina (light blue triangle); 1 from India (pink triangle); 1 from Brazil (yellow triangle); the only previous Mexican (green triangle) and 2 Mozambique sequences used as outgroup (black triangles)

Article Snippet: From 150 Bm86 cDNA tick samples sequenced by Genscript (corresponding to 600 amplicons sequenced), only 125 could be successfully assembled for the complete putative Bm86 cDNA sequence.

Techniques: Labeling